Journal: Scientific Reports

Article Title: Sesamin alleviates androgenetic alopecia by targeting the AR MAPK Wnt axis

doi: 10.1038/s41598-025-32856-4

Figure Lengend Snippet: Western blot analysis and quantitative assessment of protein expression in HaCaT cells treated with Sesamin under DHT stimulation. ( A ) Western blot images showing the expression levels of Androgen Receptor ( AR ), Bcl-2 Associated X protein ( BAX ), B-cell CLL/lymphoma 2 ( BCL2 ), Wingless-type MMTV integration site family, member 10B ( WNT10B ), Glyceraldehyde-3-phosphate Dehydrogenase ( GAPDH ), P38 Mitogen-Activated Protein Kinase (P38), and Phospho-P38 Mitogen-Activated Protein Kinase (P-P38) across different treatment groups: Untreated (UT), DHT-treated (DHT), and DHT co-treated with 10 µM or 80 µM sesamin (10, 80). GAPDH was used as a loading control. Black lines indicate where the blots were cropped, and full-length blots are provided in Supplementary Figure . ( B ) Quantification of AR expression normalized to GAPDH. ( C ) Quantification of WNT-10B expression normalized to GAPDH. ( D ) Quantification of BAX expression normalized to GAPDH. ( E ) Quantification of BCL2 expression normalized to GAPDH. ( F ) Western blot images showing the expression levels of P38 and P-P38 proteins. ( G ) Quantification of P-P38 expression normalized to total P38. Data represent mean ± SD from three independent biological experiments ( n = 3). Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparison test, which controls for multiple comparisons against a single control group. The symbols indicate comparisons to the UT group (#) or the DHT group (*). The symbols indicate the following: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Sesamin was purchased from Chengdu Aifa Biotechnology Co., Ltd.; Dulbecco’s modified eagle medium (DMEM) and fetal bovine serum were purchased from Saiaomei Cell Technology (Beijing) Co., Ltd.; dimethyl sulfoxide (DMSO) was purchased from BOSTER Biological Technology Co., Ltd.; BCA protein quantification kit was purchased from Beijing Bijie Biotechnology Co., Ltd.; MTT kit was purchased from Hangzhou Zeheng Biotechnology Co., Ltd., RIPA lysate (medium), protease inhibitor, phosphatase inhibitor were purchased from Thermo Fisher Scientific; SDS-PAGE gel preparation kit was purchased from Hangzhou Dacheng Biotechnology Co. Primary antibodies against AR , p38, phospho-p38, BAX , BCL2 , WNT10B , and GAPDH were purchased from CST Co., Ltd.; goat anti-mouse IgG, goat anti-rabbit IgG were purchased from BOSTER Biological Technology Co.

Techniques: Western Blot, Expressing, Control, Comparison

Journal: Cell Reports

Article Title: Stromal Cell-Contact Dependent PI3K and APRIL Induced NF-κB Signaling Prevent Mitochondrial- and ER Stress Induced Death of Memory Plasma Cells

doi: 10.1016/j.celrep.2020.107982

Figure Lengend Snippet:

Article Snippet: Anti-mouse BCL2, APC, REA356 , Miltenyi Biotec , Catalog # 130-105-474; RRID: AB_2651266.

Techniques: Recombinant, Electron Microscopy, Staining, Software